LOCUS       sequence-60632-e        2676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky 
            GE, Ellisman MH, Ting AY
  TITLE     Engineered ascorbate peroxidase as a genetically encoded reporter 
            for electron microscopy.
  JOURNAL   Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.
  PUBMED    23086203
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375."
COMMENT     Sequence Label: pcDNA3 Connexin43-GFP-APEX
FEATURES             Location/Qualifiers
     source          1..2676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1168..1170,1171..1173,1174..1884)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1213..1234)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1474..1493)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1821..1842
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgggtgact ggagtgcctt ggggaaatta ctggacaagg tccaagccta ctccaccgct
       61 ggagggaagg tgtggctgtc agtgctcttc atattcagaa tcctgctcct ggggacagct
      121 gttgagtcag cttggggtga tgaacagtct gcctttcgct gtaacactca acaacctggc
      181 tgcgaaaacg tctgctatga caagtccttc cccatctctc acgtgcgctt ctgggtcctt
      241 cagatcatat tcgtgtctgt gcccacactc ctgtacttgg cccatgtgtt ctatgtgatg
      301 aggaaggaag agaagctaaa caagaaagaa gaggagctca aagtggccca gactgacggg
      361 gtcaacgtgg agatgcacct gaagcagatt gaaatcaaga agttcaagta cgggattgaa
      421 gagcacggca aggtgaaaat gaggggcggc ttgctgagaa cctacatcat cagcatcctc
      481 ttcaagtctg tcttcgaggt ggccttcctg ctcatccagt ggtacatcta tgggttcagc
      541 ttgagcgcgg tctacacctg caagagagat ccctgccccc accaggtaga ctgcttcctc
      601 tcacgtccca cggagaaaac catcttcatc atcttcatgc tggtggtgtc cttggtgtct
      661 ctcgctttga acatcattga gctcttctac gtcttcttca agggcgttaa ggatcgcgtg
      721 aagggaagaa gcgatcctta ccacgccacc actggcccac tgagcccatc aaaagactgc
      781 ggatctccaa aatacgccta cttcaatggc tgctcctcac caacggctcc actctcgcct
      841 atgtctcctc ctgggtacaa gctggttact ggtgacagaa acaattcctc gtgccgcaat
      901 tacaacaagc aagctagcga gcaaaactgg gcgaactaca gcgcagagca aaatcgcatg
      961 gggcaggccg gaagcaccat ctccaactcg cacgcccagc cgttcgattt ccccgacgac
     1021 aaccagaatg ccaaaaaagt tgctgctgga catgaactcc agccattagc catcgtggac
     1081 caacgacctt ccagcagagc cagcagccgc gccagcagca ggcctcggcc tgatgacctg
     1141 gagattggat ccgggggtgg tgggtctatg gtgagcaagg gcgaggagct gttcaccggg
     1201 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1261 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1321 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1381 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1441 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1501 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1561 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1621 tatatcatgg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     1681 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1741 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1801 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1861 ctcggcatgg acgagctgta caaggggcgc gccaagggct cgggctcgac ctcgggctcg
     1921 ggcaggggaa aatcttaccc aactgttagt cccgattacc aggacgccat tgaaaaggct
     1981 aagaggaagc tcagaggttt tatcgctgag aagaaatgcg ctcctctaat tctccgtttg
     2041 gcattccact ctgctggtac ttttgattcc aagacaaaga ctggtggtcc tttcggaaca
     2101 attaagcacc aagctgagct tgctcatggt gctaacaacg gtcttgatat cgcggttagg
     2161 ctgttggagc ctattaagga gcaattccct attgtgagct atgctgattt ctaccagttg
     2221 gctggtgttg ttgctgttga gattaccggt ggacctaagg ttcctttcca ccctggtagg
     2281 gaggacaagc ctgagccacc acctgagggt cgcttgcctg atgccactaa gggttctgac
     2341 catttgaggg atgtgtttgg aaaggctatg gggcttagtg atcaggacat tgttgctcta
     2401 tctggtggtc acaccattgg agctgcacac aaggagcgtt ctggatttga gggaccgtgg
     2461 acttctaatc ctctcatttt tgacaactca tatttcactg agttgttgac tggtgagaag
     2521 gatggccttc ttcagttgcc aagtgataag gcacttttga ctgactctgt attccgccct
     2581 cttgttgaga aatatgctgc ggatgaagat gttttctttg ctgattatgc tgaagcacat
     2641 cttaagctct ctgagcttgg atttgctgaa gcctaa
//
