LOCUS       sequence-60668-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FLJ10324A
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             611..628
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(681..699)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      695..742
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(866..885)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatggacc
      121 ccgccgagct ctccacccag ctgtcggccc ctggtgtcct gaaggtgttt ggggacagtg
      181 tctgcacagg aacccactac aagagcgtcc tggccaccgg cacctccagc gcccgtgagc
      241 tggtgaagga ggcgctggag cggtacgccc tggaccccag gcaggccggc cagtacgtgc
      301 tgtgtgacgt ggtgggccaa gccggcgatg ctgggcagcg gtggcaggcc cggtgctttc
      361 gggtgtttgg ggacagtgag aagcccctct tgatccagga attatggaaa ccccgagaag
      421 gtttatcccg gaggtttgag ctgaggaaga ggtcggacgt ggaggagctg gcagccaagg
      481 aggtggacac catcacggca gggataaacg cccaggcccg gaggctgcag cggagtcgcg
      541 cgaagtgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca agcttgcggc
      601 cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
      661 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
      721 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
      781 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      841 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      901 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      961 gctttacg
//
