LOCUS       sequence-60681-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDX5A
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             902..919
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgcgca
      121 cagcacaaga ggtggaaaca tacagaagaa gcaaggaaat tacagttaga ggtcacaact
      181 gcccgaagcc agttctaaat ttttatgaag ccaatttccc tgcaaatgtc atggatgtta
      241 ttgcaagaca gaatttcact gaacccactg ctattcaagc tcagggatgg ccagttgctc
      301 taagtggatt ggatatggtt ggagtggcac agactggatc tgggaaaaca ttgtcttatt
      361 tgcttcctgc cattgtccac atcaatcatc agccattcct agagagaggc gatgggccta
      421 tttgtttggt gctggcacca actcgggaac tggcccaaca ggtgcagcaa gtagctgctg
      481 aatattgtag agcatgtcgc ttgaagtcta cttgtatcta cggtggtgct cctaagggac
      541 cacaaatacg tgatttggag agaggtgtgg aaatctgtat tgcaacacct ggaagactga
      601 ttgacttttt agagtgtgga aaaaccaatc tgagaagaac aacctacctt gtccttgatg
      661 aagcagatag aatgcttgat atgggctttg aaccccaaat aaggaagatt gtggatcaaa
      721 taagacctga taggcaaact ctaatgtgga gtgcgacttg gccaaaagaa gtaagacagc
      781 ttgctgaaga tttcctgaaa gactatattc atataaacat tggtgcactt gaactgtgac
      841 agtaaaggtg gatacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga
      901 gcaccaccac caccaccact gagatccggc tg
//
