LOCUS       sequence-60683-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DCTDA
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(98..115)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             671..693
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    708..732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(732..751)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(733..751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(835..854)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctcaatg gtggtgatga tggtgcgcac
      121 tcggtctgct gttaattgaa tcaaagtcaa tgacaatctt gctgcacttc ggtatgaatt
      181 tccggaatgt caccccggcc atattaaaca ggagcctcgc agcagttgcc tcgtcactat
      241 catggtattt atcagacatg aaaatcactt cttttatacc tgcctggatg atgagcttag
      301 cgcattcatt acaagggaac aaggcgacat acatactaca gcctttcaca tcggtcaaat
      361 ttttgttcat gatggcattc agctccgcat ggcacacgta cgggtatttg gtgtccagct
      421 tattctctgc tgtccttctc caaggcaaca cgtcatcact gcacccattt ggcatcccat
      481 tgtacccaat cccgacaatc ttgttttctg aattcacgat gcaggcgccg acctgggaat
      541 ttggatcttt gcttctctgt gctgataaga aggccacagc cataaaatac tctggccatt
      601 ccaaatagtc gtcccgtttc ttgcaggaca ttaattatct cctctttgtg attcatgatt
      661 catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga attgttatcc
      721 gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct
      781 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
      841 tcgccgacat caccgatggg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
      901 tttcggcgtg gggtatggtg gcaggccccg tggccggggg gactgttggg cgccatctct
      961 tgca
//
