LOCUS       sequence-60687-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LMSAHA
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatggcgg
      121 actacaaggt aaaagacatc agcctcgcgg agtggggccg caaggcgatc gagctcgcgg
      181 aaaacgagat gcccggtctg atggagctgc gccgcgagta cggaccctcc cagccgctga
      241 agggcgccaa gattgccggc tgcctgcaca tgaccgtaca gactgccgtg ctgatcgaga
      301 ccctcaaggc cctcggtgcg gagctgcgct ggtcgtcgtg caacatcttt tccactcagg
      361 ataacgccgc tgcggccatt gccaagactg gcgtaccggt gtttgcgtgg aagggtgaga
      421 cagacgagga gtatgagtgg tgcatcgccc agacagtgaa gggcttcagc ggtgacggtc
      481 tgccgaacat gatcctcgac gacggtggcg acctcacgaa tctggtgatc gaccgctacc
      541 ccgagctcgt gcccaagatc ttcggtatct ccgaggagac cacgacgggt gtgaagaacc
      601 tgtacaagcg cctgagcaag ggcaacctcc ccatctccgc catcaacgtt aacgacagcg
      661 tgacgaagag caagttcgac aacctttacg gctgccgaga gtccttggtg gacggcatca
      721 agcgcgccac ggatgtcatg attgccggca agacgtgctg cgtgtgcgga tacggtgatg
      781 ttggtaaggg ctgcgccgcc gccctgcgcg ccttcggcgc tcgcgttgtg gtaacggagg
      841 tggaccctat caatgccctt caggcctcca tggagggcta ccaggtcgct ctggtcgaag
      901 acgtcatggc cgatgcgcac atcttcgtga cgaccac
//
