LOCUS       sequence-60724-e        5034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1273
FEATURES             Location/Qualifiers
     source          1..5034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    37..57
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    57..164
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     81..97
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     81..97
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(131..147)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(132..148)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(164..184)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(192..209)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(192..208)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(214..225)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      2170..2493
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2637..2656)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2756..2778
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2816..2834)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2902..3006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3007..3075,3076..3867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3225..3244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4038..4626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4527..4546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgagc
       61 tccaccgcgg tggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgata
      121 tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccaacttt attatacata
      181 gttgataatt cactggccgt cgttttacat ggcacgccct tctattacag ggtttctgat
      241 aacaaaccgg ttattaactc ccaacaaggg atgatttcaa ttcatcatgc tcaaattgac
      301 ccaaattaag ttacatgaca aattcatcgc ccttttcaac tcttcttggc tcatcatctg
      361 ttattctgtt cctattctgt ctgcacccta tacccctttg catactctct cgtcattcct
      421 ttcttggata gtgtcttctt ctccccagct ctgctacttc tatgacttgc gcccgctgct
      481 tttccgctcg ttcctctcct ctctgacgtc ttcttctgct tcttttctcc ttcctttttc
      541 ccttctcaat cttcctcttt ttcccatttc tgtcaatcat tcgaagaaga agaagaagaa
      601 gaccctcatt catttcattt ttttttctgt cggtgtgtgc tgctcggtta agagtgagct
      661 ctctattcca cgtcttcttc tttttcttct tcgattcgaa tcaatcactc caccaaaacg
      721 cattcgtttt tgggattcac cccgggtgcc ggatctgcgg cttactcacc agacctggcc
      781 ccatccgatt accacctatt cgcttcgatg ggacacgcac tcgctgagca gcgcttcgat
      841 tcttacgaaa gtgtgaaaaa atggctcgat gaatggttcg ccgcaaaaga cgatgagttc
      901 tactggcgtg gaatccacaa attgcccgag agatgggaaa aatgtgtagc tagcgacggc
      961 aaatactttg aataaatgat tttttctttt tccacaaaat ttaacgtgtt ttttgattta
     1021 aaaaaaacga catttcatac ttgtacacct gatatctaga tacctaggtg agctctggta
     1081 cccccctggc gttacccaac ttaatcgcct tgcagcagag tcgtattacg tagcccggcc
     1141 tgcaggtcga ctctagagga tcaccaaaaa cggaacgttg agctggacgg aaatagtggt
     1201 aaagtgacat gattatagtt tgaagatttc taatttcaca attagagcaa atgttgttcg
     1261 gtatttattt tcaacggtat ttatactatt ttccaccttt ttctagaaca ttcgagctgc
     1321 ttgttgcaaa aggagggcga ctcacattcg gtacatggaa aagtagtgta cacaataaag
     1381 agacccagat acattttccg tctgcgtctc tttgcaccca ccgggagtat tttcaaacga
     1441 atgcatctag gaccttctag aacattctgt aaggctgcag aatgcgggta tataaggaaa
     1501 gcgggctcag aggaagccaa cacgctttgt tctagtgcat ctaaaaaact tcgaaaatcc
     1561 tcatcggatc ctatcgattc gcggccgctg tacacccggg tgccctatag tgagtcgtat
     1621 tggtaccggt agaaaaaata tgcgctttga tttccaaaac ttaaaatttt cgatgatttt
     1681 catatttttg tggaacatca tagtcggatt tttacttgct cttgtaaaga ttttcaaaat
     1741 ctacatgtat cttgatctgc ctgaaaatgt atgggtaaat cggtttgcgc atgttattgc
     1801 tcttattgga tcaattgctt cattgtggtt aatttttttt agcccatttg aaattaagca
     1861 aggaagattt tctgtctcgt caaaaactat cctgcttctt gtttgcaagg gtttgattgg
     1921 cttgatattt cttgcacaga ttttcgccca tgtcctttac attgacccat gccagaatat
     1981 gctcttatca ttgaactctg cactgttctc cttgctgctg aattattatg agtctcgtgg
     2041 tgcgatccat ccgttgatgg aattagttca aacagctggc ctgagaccaa ctttgtgcgc
     2101 tgctgtacga tttaattgtc tcaactcaac tgctcatatt gatccaagtg ttgaaaatcc
     2161 atgagataaa tgcaaaatcc tttcaagcat tcccttcttc tctatcactc ttctttcttt
     2221 ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt ttatgacttt
     2281 ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat gtgattctgt
     2341 ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa cccgaaaggt
     2401 tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc caacaaaaag
     2461 aggaaacttt attgtgccgc caagaaaaaa gtctcaagct ggcgtaatag cgaagaggcc
     2521 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     2581 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     2641 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     2701 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     2761 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     2821 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     2881 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2941 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3001 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3061 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3121 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3181 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3241 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3301 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3361 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3421 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3481 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3541 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3601 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3661 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3721 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3781 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3841 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3901 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3961 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4021 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4081 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4141 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4201 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4261 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4321 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4381 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4441 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4501 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4561 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4621 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg tcttttgctg gccttttgct
     4681 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4741 tgagctgata ccgcaccgta cgtctcgagg aattctatca ggtgtacaag tatgaaatgt
     4801 cgtttcgaac atatagatgt ctcgcaaacg taaatattta tcgattgtca taaaactttg
     4861 accttgtgaa gtgtcaacct tgactgtcga accaccatag tttggcgcga attgagcgtc
     4921 ataattgttt actctcagtg cagtcaacat gtcgagtttc gtgccgaata aagagcaaac
     4981 gcggacagta ttaattttct gttttcattt gaagaaaaca gctgcggccg gatc
//
