LOCUS       sequence-60740-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  TITLE     Plasmids to assay for nuclease cleavage in planta
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHEX2 QQR ZFN
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             315..902
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINTNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gtacaaaaaa gcaggctatg gaaaaactgc ggaacggatc cggggaccct ggcaaaaaga
       61 aacagcacgc gtgtccggaa tgcggcaagt cctttagtca gtctagcaac ctgcagaagc
      121 atcaacgtac gcataccggg gaaaaacctt acaaatgtcc ggaatgcggc aagtccttta
      181 gtcagtctag caacctgcag aagcatcaac gtacgcatac cggggaaaaa ccttacaaat
      241 gtccggaatg cggcaagagc tttagtagaa gtgatcatct gtcaagacat caacgtacgc
      301 atcagaataa gaagcaacta gtcaaaagtg aactggagga gaagaaatct gaacttcgtc
      361 ataaattgaa atatgtgcct catgaatata ttgaattaat tgaaattgcc agaaattcca
      421 ctcaggatag aattcttgaa atgaaggtaa tggaattttt tatgaaagtt tatggatata
      481 gaggtaaaca tttgggtgga tcaaggaaac cggacggagc aatttatact gtcggatctc
      541 ctattgatta cggtgtgatc gtggatacta aagcttatag cggaggttat aatctgccaa
      601 ttggccaagc agatgaaatg caacgatatg tcgaagaaaa tcaaacacga aacaaacata
      661 tcaacactaa tgaatggtgg aaagtctatc catcttctgt aacggaattt aagtttttat
      721 ttgtgagtgg tcactttaaa ggaaactaca aagctcagct tacacgatta aatcatatca
      781 ctaattgtaa tggagctgtt cttagtgtag aagagctttt aattggtgga gaaatgatta
      841 aagccggcac attaacctta gaggaagtga gacggaaatt taataacggc gagataaact
      901 tttaaaccca gctttctt
//
