LOCUS       sequence-60897-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY
  TITLE     Proteomic Mapping of Mitochondria in Living Cells via Spatially 
            Restricted Enzymatic Tagging.
  JOURNAL   Science. 2013 Jan 31.
  PUBMED    23371551
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 31."
COMMENT     Sequence Label: pcDNA3-APX(W41F)-NES
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..73)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             complement(80..826)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQKAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPEVPFHPGREDKPEPPPEGRLPDATKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             complement(827..850)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      850..859
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gaactataga atagggccct ctagatgcat gctcgagcta ttagtccagg gtcaggcgct
       61 ccaggggggg cagctgcagg gcatcagcaa acccaagctc ggaaagcttt tggtgagcct
      121 cagcgtaatc agcaaagaag gcatcttcgt ccgctgcata tttatcaacg agagggcgga
      181 atacagggtc agacaaaaga gccttgtcag aaggtagctg aaggagacct tccttctcac
      241 cactcaacaa ctccgtgaag tatgagttgt cgaaaataag aggattagag gtccagggac
      301 cctcaaatcc agaacgctcc ttgtgtgcag ctccaatagt gtgaccccca gatagagcaa
      361 cgatatcttg gtcagtaagc cccatagctt tgccaaacac atctctcaaa tggtcagaac
      421 ccttagtggc atcgggcaag cgaccctctg gtggtggctc aggcttgtcc tctcttccag
      481 ggtggaatgg aacttcaggt ccacccgtga cctcaacggc aacaacgcca gccaactggt
      541 agaaatcggc gtagctcaaa atagggaact ccgccttgag tggctccaaa agcctaacag
      601 cgatgtcaag accgttgtta gcgctgtgag ccagttcggc agggtgcttg atggttccga
      661 agggtccacc ggtcttcgtg cccttgtcaa aggttccagc agagtggaat gccaaacgga
      721 gcattagagg agcgcatctc ttctcagcga tgaagcctct gagcttcttc ttcgccttct
      781 caacggcctt ctggtaatca gcactcacag ttgggtaaga ctttccctta tcgtcgtcat
      841 ccttgtagtc catggtggcg gccgcaagct tgggtct
//
