LOCUS       sequence-60930-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Shen RR, Zhou AY, Kim E, Lim E, Habelhah H, Hahn WC
  TITLE     IkappaB kinase epsilon phosphorylates TRAF2 to promote mammary 
            epithelial cell transformation.
  JOURNAL   Mol Cell Biol. 2012 Dec;32(23):4756-68. doi: 10.1128/MCB.00468-12. 
            Epub 2012 Sep 24.
  PUBMED    23007157
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00468-12"; journalName: "Mol Cell Biol"; date: 
            "2012-12"; volume: "32"; issue: "23"; pages: "4756-68"
COMMENT     Sequence Label: pLKO-shCIAP2-A
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    110..227
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        276..759
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     705..723
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 aaggacgaaa caccggcagt tcgtacattt ctttcatctc gagatgaaag aaatgtacga
       61 actgttttta attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa
      121 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
      181 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
      241 gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa
      301 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
      361 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
      421 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
      481 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
      541 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
      601 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
      661 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
      721 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cga
//
