LOCUS       sequence-60965-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Ajith Karunarathne WK, O'Neill PR, Martinez-Espinosa PL, 
            Kalyanaraman V, Gautam N
  TITLE     All G protein betagamma complexes are capable of translocation on 
            receptor activation.
  JOURNAL   Biochem Biophys Res Commun. 2012 May 11;421(3):605-11. doi: 
            10.1016/j.bbrc.2012.04.054. Epub 2012 Apr 19.
  PUBMED    22538369
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2012.04.054"; journalName: "Biochem Biophys Res 
            Commun"; date: "2012-05-11- 11"; volume: "421"; issue: "3"; pages: 
            "605-11"
COMMENT     Sequence Label: Venus-G protein Gamma 5
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(231..689,690..692,693..695))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     370..389
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     629..650
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(739..758)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(740..758)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(803..854)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(832..852)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tctagactac aaaaggagca gactttctgg ggcctgaagg gatttgtact tgaagatact
       61 ccagtcagca gagggtcatg ttgagcattc tgcagacaga actgtttcaa atctgcagct
      121 gcctgggaaa ccttcacccg attgagcccg gcctccagcc ggagctgttg aaccactttt
      181 ttcatagccg cgacgctaga agaacccgac atgcctccac cggatccgcc ggcggtgata
      241 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
      301 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
      361 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
      421 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg
      481 aagcactgca ggccgtagcc cagggtggtc acgagggtgg gccagggcac gggcagcttg
      541 ccggtggtgc agatcagctt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      601 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggtacc
      661 accccggtga acagctcctc gcccttgctc accatggtgg taccaagctt aagtttaaac
      721 gctagccagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag
      781 tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac
      841 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatt
//
