LOCUS       sequence-60967-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-PTD004
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(44..58,59..64,65..81,82..88)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    64..80
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             131..142
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             155..175
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             200..220
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aaataggcgt atcacgaggc cctttcgtct tcacctcgag aaatcataaa aaatttattt
       61 gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac
      121 agaattcatt aaagaggaga aattaactat gaaacatcac catcaccatc accatagcga
      181 ttacgacatc cccactactg agaatcttta ttttcaggga tcccccccta aaaagggagg
      241 tgatggaatt aaaccacccc caatcattgg aagatttgga acctcactga aaattggtat
      301 tgttggattg ccaaatgttg ggaaatctac tttcttcaat gtgttaacca atagtcaggc
      361 ttcagcagaa aacttcccgt tctgcactat tgatcctaat gagagcagag tacctgtgcc
      421 agatgaaagg tttgactttc tttgtcaata ccacaaacca gcaagcaaaa ttcctgcctt
      481 tctaaatgtg gtggatattg ctggccttgt gaaaggagct cacaatgggc agggcctggg
      541 gaatgctttt ttatctcata ttagtgcctg tgatggcatc tttcatctaa cacgtgcttt
      601 tgaagatgat gatatcacgc acgttgaagg aagtgtagat cctattcgag atatagaaat
      661 aatacatgaa gagcttcagc ttaaagatga ggaaatgatt gggcccatta tagataaact
      721 agaaaaggtg gctgtgagag gaggagataa aaaactaaaa cctgaatatg atataatgtg
      781 caaagtaaaa tcctgggtta tagatcaaaa gaaacctgtt cgcttctatc atgattggaa
      841 tgacaaagag attgaagtgt tgaataaaca cttatt
//
