LOCUS       sequence-60972-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Ajith Karunarathne WK, O'Neill PR, Martinez-Espinosa PL, 
            Kalyanaraman V, Gautam N
  TITLE     All G protein betagamma complexes are capable of translocation on 
            receptor activation.
  JOURNAL   Biochem Biophys Res Commun. 2012 May 11;421(3):605-11. doi: 
            10.1016/j.bbrc.2012.04.054. Epub 2012 Apr 19.
  PUBMED    22538369
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2012.04.054"; journalName: "Biochem Biophys Res 
            Commun"; date: "2012-05-11- 11"; volume: "421"; issue: "3"; pages: 
            "605-11"
COMMENT     Sequence Label: Venus-Gprotein Gamma 9
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(230..688,689..691,692..694))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     369..388
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     628..649
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(738..757)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(739..757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(802..846)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 atcagcttat tatacaaccg cctttttcct tgaagggatt cttgtcctca gggatgcctt
       61 taagaagagg gtcgtttcct gcttcagcct ccacataatc cttgatttcc tttcctgttt
      121 tggaaatcag agctcttggg ttcttcactt ccttcttcag ctgctctacc tccatcttta
      181 agagttcctt ctcgctgagc tcttgggcca tgcctccacc ggatccgccg gcggtgatat
      241 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
      301 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
      361 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
      421 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga
      481 agcactgcag gccgtagccc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
      541 cggtggtgca gatcagcttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
      601 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggtacca
      661 ccccggtgaa cagctcctcg cccttgctca ccatggtggt accaagctta agtttaaacg
      721 ctagccagct tgggtctccc tatagtgagt cgtattaatt tcgataagcc agtaagcagt
      781 gggttctcta gttagccaga gagctctgct tatatagacc tcccaccgta cacgcctacc
      841 gcccatttg
//
