LOCUS       sequence-61247-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Bird LE
  TITLE     High throughput construction and small scale expression screening of
            multi-tag vectors in Escherichia coli.
  JOURNAL   Methods. 2011 Sep;55(1):29-37. Epub 2011 Aug 10.
  PUBMED    21856427
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2011-09"; volume: "55(1)"; pages: "29-37. Epub 2011 Aug 10"
COMMENT     Sequence Label: pOPINNUSA
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..117
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             138..155
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tatccgggac ctttaattca acccaacaca atatattata gttaaataag aattattatc
       61 aaatcatttg tatattaatt aaaatactat actgtaaatt acattttatt tacaatcaaa
      121 ggagatatac catggcacac catcaccacc atcacaacaa agaaattttg gctgtagttg
      181 aagccgtatc caatgaaaag gcgctacctc gcgagaagat tttcgaagca ttggaaagcg
      241 cgctggcgac agcaacaaag aaaaaatatg aacaagagat cgacgtccgc gtacagatcg
      301 atcgcaaaag cggtgatttt gacactttcc gtcgctggtt agttgttgat gaagtcaccc
      361 agccgaccaa ggaaatcacc cttgaagccg cacgttatga agatgaaagc ctgaacctgg
      421 gcgattacgt tgaagatcag attgagtctg ttacctttga ccgtatcact acccagacgg
      481 caaaacaggt tatcgtgcag aaagtgcgtg aagccgaacg tgcgatggtg gttgatcagt
      541 tccgtgaaca cgaaggtgaa atcatcaccg gcgtggtgaa aaaagtaaac cgcgacaaca
      601 tctctctgga tctgggcaac aacgctgaag ccgtgatcct gcgcgaagat atgctgccgc
      661 gtgaaaactt ccgccctggc gaccgcgttc gtggcgtgct ctattccgtt cgcccggaag
      721 cgcgtggcgc gcaactgttc gtcactcgtt
//
