LOCUS       sequence-61279-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Lee JH, Silhavy JL, Lee JE, Al-Gazali L, Thomas S, Davis EE, Bielas 
            SL, Hill KJ, Iannicelli M, Brancati F, Gabriel SB, Russ C, Logan CV,
            Sharif SM, Bennett CP, Abe M, Hildebrandt F, Diplas BH, Attie-Bitach
            T, Katsanis N, Rajab A, Koul R, Sztriha L, Waters ER, Ferro-Novick 
            S, Woods CG, Johnson CA, Valente EM, Zaki MS, Gleeson JG
  TITLE     Evolutionarily assembled cis-regulatory module at a human ciliopathy
            locus.
  JOURNAL   Science. 2012 Feb 24;335(6071):966-9. Epub 2012 Jan 26.
  PUBMED    22282472
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2012-02-24"; volume: "335(6071)"; pages: "966-9. Epub 2012 
            Jan 26"
COMMENT     Sequence Label: TMEM216-mCherry
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(831..850)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(832..850)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(895..939)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
       61 actccttgat gatggccatg ttatcctcct cgcccttgct caccatacgc gtaatcgtgt
      121 ccatactgga gaaagcagcc aaggtgagca cctcaagtaa aagctctgag ccacagaaga
      181 agagcaagat gccattcatg atggcttcca ggcggagtac gtaggtctgc agcagcaggt
      241 aataggaggc catcatggca gatgggaagg tcaaggccac gctaatactg agtggcatct
      301 ttcgctggca gaggtttccc tttgtaccaa aaaacaggcg aattacttca attccaagat
      361 aaaggaggag catcaccaca tccagtacta ggttagctgt tggatatggt agcaggacac
      421 ctttatacag aaatatgaaa agttccagca ggaaataggt agcattatac cacccgttca
      481 gaaagaacag gatttccagc ggggtggagg acaaccgttt acctcgcggc gccatcttca
      541 gtccccgtgg cagcatacgc tgccacagcg gctcccggag cagcgcggcg ctgccggaaa
      601 cagggacgaa gcggcctggg tttccggccc ctctgcccgc ccgcggcact ctgtggtcac
      661 ccggagctgg acgagctcgg gcctggccgc aagtggccct cgctcggatg tcccctctcc
      721 agacctaagg gataacggtg accacccaca agggcgaatt ccaccacact ggactagtgg
      781 atccgagctc ggtaccaagc ttaagtttaa acgctagcca gcttgggtct ccctatagtg
      841 agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct
      901 gcttatatag acctcccacc gtacacgcct accgcccatt tgc
//
