LOCUS       sequence-61428-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  TITLE     TLR Signaling Plasmids
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-TRIF-CFP
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             274..285
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccatggc ctgcacaggc ccatcacttc ctagcgcctt
      121 cgacattcta ggtgcagcag gccaggacaa gctcttgtat ctgaagcaca aactgaagac
      181 cccacgccca ggctgccagg ggcaggacct cctgcatgcc atggttctcc tgaagctggg
      241 ccaggaaact gaggccagga tctctctaga ggcattgaag gccgatgcgg tggcccggct
      301 ggtggcccgc cagtgggctg gcgtggacag caccgaggac ccagaggagc ccccagatgt
      361 gtcctgggct gtggcccgct tgtaccacct gctggctgag gagaagctgt gccccgcctc
      421 gctgcgggac gtggcctacc aggaagccgt ccgcaccctc agctccaggg acgaccaccg
      481 gctgggggaa cttcaggatg aggcccgaaa ccggtgtggg tgggacattg ctggggatcc
      541 agggagcatc cggacgctcc agtccaatct gggctgcctc ccaccatcct cggctttgcc
      601 ctctgggacc aggagcctcc cacgccccat tgacggtgtt tcggactgga gccaagggtg
      661 ctccctgcga tccactggca gccctgcctc cctggccagc aacttggaaa tcagccagtc
      721 ccctaccatg cccttcctca gcctgcaccg cagcccacat gggcccagca agctctgtga
      781 cgacccccag gccagcttgg tgcccgagcc tgtccccggt ggctgccagg a
//
