LOCUS       sequence-61432-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Christofk HR, Vander Heiden MG, Harris MH, Ramanathan A, Gerszten 
            RE, Wei R, Fleming MD, Schreiber SL, Cantley LC
  TITLE     The M2 splice isoform of pyruvate kinase is important for cancer 
            metabolism and tumour growth.
  JOURNAL   Nature. 2008 Mar 13;452(7184):230-3. doi: 10.1038/nature06734.
  PUBMED    18337823
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2008-03-13- 13"; volume: "452"; issue: "7184"; 
            pages: "230-3"
COMMENT     Sequence Label: pLHCX-FLAG-mPKM2
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             72..95
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc aagcttgtta
       61 acgccaccat ggactacaaa gacgatgacg ataaagcaag ggatccacta gtaacggccg
      121 ccagtgtgct ggaattcatg ccgaagccac acagtgaagc agggactgcc ttcattcaga
      181 cccagcagct ccatgcagcc atggctgaca ccttcctgga acacatgtgc cgcctggaca
      241 ttgactctgc ccccatcacg gcccgcaaca ctggcatcat ttgtaccatt gggcctgctt
      301 cccgatctgt ggagatgctg aaggagatga ttaagtctgg aatgaatgtg gctcggctga
      361 atttctctca tggaacccat gagtaccatg cagagaccat caagaatgtc cgtgaagcca
      421 cagaaagctt tgcatctgat cccattctct accgtcctgt tgcggtggct ctggatacaa
      481 agggacctga gatccggact ggactcatca agggcagcgg caccgctgag gtggagctga
      541 agaagggagc cactctgaag atcaccctgg acaacgctta catggagaag tgtgacgaga
      601 acatcctgtg gctggactac aagaacatct gcaaggtggt ggaggtgggc agcaagatct
      661 acgtggacga tgggctcatc tcactgcagg tgaaggagaa aggcgctgac ttcctggtga
      721 cggaggtgga gaatggtggc tccttgggca gcaagaaggg cgtgaacctg ccgggcgctg
      781 ctgtggatct ccccgctgtg tcggaaaagg acatccagga cctgaagttt ggggtggagc
      841 aggatgtgga catggtgttt gcatctttca tccgcaaggc agccgacgtg catgaagtca
      901 ggaaggtgct gggagagaag ggcaagaaca tcaagatcat cagcaaaatc gagaaccatg
      961 a
//
