LOCUS       sequence-61480-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-AGTG
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(600..618)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(601..618)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(636..652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(636..652)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(649..671)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    660..676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(684..690,691..708,709..714))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    729..750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgaattggg ccctctagat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga
       61 attcgccctt cgtcctagcg cgtctcggct caccccagag caggtcgtgg caatcgcctc
      121 caacattggc gggaaacagg cactcgagac tgtccagcgc ctgcttcccg tgctgtgcca
      181 agcgcacgga ctcaccccag agcaggtcgt ggcaatcgcg agcaataacg gcggaaaaca
      241 ggctttggaa acggtgcaga ggctccttcc agtgctgtgc caagcgcacg gcctcacccc
      301 agagcaggtc gtggccattg cctcgaatgg agggggcaaa caggcgttgg aaaccgtaca
      361 acgattgctg ccggtgctgt gccaagcgca cggattaacc ccagagcagg tcgtggcaat
      421 cgcgagcaat aacggcggaa aacaggcttt ggaaacggtg cagaggctcc ttccagtgct
      481 gtgccaagcg cacggactca gagacgtggg cacaggaaag aagggcgaat tccagcacac
      541 tggcggccgt tactagtgga tccgagctcg gtaccaagct tgatgcatag cttgagtatt
      601 ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      661 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      721 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      781 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      841 ttgcgtattg ggcgct
//
