LOCUS       sequence-61598-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-CATG
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(596..614)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(597..614)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(632..648)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(632..648)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(645..667)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    656..672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(680..686,687..704,705..710))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    725..746
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(864..881)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgggcgatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattcgcc cttgccgtcc tagcgcgtct cggctcaccc cagagcaggt cgtggcgatc
      121 gcaagccacg acggaggaaa gcaagccttg gaaacagtac agaggctgtt gcctgtgctg
      181 tgccaagcgc acggactcac cccagagcag gtcgtggcaa tcgcctccaa cattggcggg
      241 aaacaggcac tcgagactgt ccagcgcctg cttcccgtgc tgtgccaagc gcacggcctc
      301 accccagagc aggtcgtggc cattgcctcg aatggagggg gcaaacaggc gttggaaacc
      361 gtacaacgat tgctgccggt gctgtgccaa gcgcacggat taaccccaga gcaggtcgtg
      421 gcaatcgcga gcaataacgg cggaaaacag gctttggaaa cggtgcagag gctccttcca
      481 gtgctgtgcc aagcgcacgg actcagagac gtgggcaagg gcgaattcca gcacactggc
      541 ggccgttact agtggatccg agctcggtac caagcttgat gcatagcttg agtattctat
      601 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      661 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      721 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      781 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      841 gtattgggcg ctcttccgct ttcctcgctc actgactcgc tgc
//
