LOCUS       sequence-61641-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-ACGT
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(587..605)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(588..605)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(623..639)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(623..639)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(636..658)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    647..663
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt cgcccttcgt
       61 cctagcgcgt ctcggctcac cccagagcag gtcgtggcaa tcgcctccaa cattggcggg
      121 aaacaggcac tcgagactgt ccagcgcctg cttcccgtgc tgtgccaagc gcacggactc
      181 accccagagc aggtcgtggc gatcgcaagc cacgacggag gaaagcaagc cttggaaaca
      241 gtacagaggc tgttgcctgt gctgtgccaa gcgcacggcc tcaccccaga gcaggtcgtg
      301 gcaatcgcga gcaataacgg cggaaaacag gctttggaaa cggtgcagag gctccttcca
      361 gtgctgtgcc aagcgcacgg attaacccca gagcaggtcg tggccattgc ctcgaatgga
      421 gggggcaaac aggcgttgga aaccgtacaa cgattgctgc cggtgctgtg ccaagcgcac
      481 ggactcagag acgtgggcac aggaaagaag ggcgaattcc agcacactgg cggccgttac
      541 tagtggatcc gagctcggta ccaagcttga tgcatagctt gagtattcta tagtgtcacc
      601 taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      661 caattccaca caacatacga gccggaagca ta
//
