LOCUS       sequence-61654-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Lemaire PA, Lary J, Cole JL
  TITLE     Mechanism of PKR activation: dimerization and kinase activation in 
            the absence of double-stranded RNA.
  JOURNAL   J Mol Biol. 2005 Jan 7;345(1):81-90.
  PUBMED    15567412
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-7"; volume: "345(1)"; pages: "81-90"
COMMENT     Sequence Label: pPET-PKR/PPase
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             755..777
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgaagc
       61 ttgtcgacgg agctcgaatt ctcatgcgcc ttctccctgt acctgaatca atgttaggtt
      121 tccgcagaac actgcgccgg tatcgatata catttggttg gcaaacttga gtggtttcac
      181 tgctggcgta tgaccaaaga tgaacgtgtc cgcgcctttg atttctttca cgatcccgtt
      241 ttgtgagttg ctgattcgtt cgcggttcca gattacctgc tgatgatcaa ctggctttcc
      301 aaactcgtat tcgtcaaagg gataatcggc gtggcagata acatattttt tatctttgct
      361 caccagttcg atgattaacg gaagttcatc tgctttatgg gcaagagctt tagccagaat
      421 ttctttgtcg taatcgagat taaagaacca gccaccgcca ttaagcagcc agtgattaac
      481 gtttccacgc tctgataagc catcaatcat catttgctca tggtttccac gtacagctct
      541 gaaccagggg aatgtgatta attccaggca ttcaacgttc tctgcaccac gatcaaccaa
      601 atcgcccacc gagataagca ggtctttttt gttgtcgaat ccaatcgtat ccagtttgtt
      661 catcaggttc gtgtagcatc cgtgcagatc gccaactacc caaatatttc ggtatttgct
      721 gccatcaatt ttttcgtaat agcgcatatg tatatctcct tcttaaagtt aaacaaaatt
      781 atttctagtc gagtgcggcc gcaagcttgt cgacggagct cgaattgagg cagatcgtca
      841 gtcagtcacg atgaattccc ggggatcctt cagaagggct ctaacatgtg tgtcgttcat
      901 ttttctctgg gcttttcttc
//
