LOCUS       sequence-62055-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTF257 (pFA6a-TEV-6xGly-His3MX)
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..54
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      113..300
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(168..192)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        375..718
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacgagaatt tatacttcca aggttctggt
       61 ggaggcggtg gaggctctag aggtaccctc gaggcgcgcc acttctaaat aagcgaattt
      121 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt
      181 ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta
      241 ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca cctctaccgg
      301 cagatccgct agggataaca gggtaatata gatctgttta gcttgcctcg tccccgccgg
      361 gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca
      421 gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat
      481 catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc
      541 tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg
      601 cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac
      661 ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat
      721 ggcagaacca gcccaaaaaa agcaaaaaca aactgttcag gagcgcaagg cgtttatctc
      781 ccgtatcact aatgaaacta aaattcaaat cgctatttcg ctgaatggtg gttatattca
      841 aataaaagat tcgattcttc ctgcaaagaa ggatgacgat gtagcttccc aagctactca
      901 gtcacaggtc atcga
//
