LOCUS       sequence-62064-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pZC06 (pFA6a-PSc-6xGly-PrtA-KanMX)
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..57
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             115..288
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             289..462
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     terminator      521..708
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(576..600)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacttggaag tattgttcca aggtccaggt
       61 ggaggcggtg gaggctctag aaaaaccgcg gctcttgcgc aacacgatga agccgtggac
      121 aacaaattca acaaagaaca acaaaacgcg ttctatgaga tcttacattt acctaactta
      181 aacgaagaac aacgaaacgc cttcatccaa agtttaaaag atgacccaag ccaaagcgct
      241 aaccttttag cagaagctaa aaagctaaat gatgctcagg cgccgaaagt agacaacaaa
      301 ttcaacaaag aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa
      361 gaacaacgaa acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt
      421 ttagcagaag ctaaaaagct aaatggtgct caggcgccga aagtagacgc gaattccgcg
      481 gggaagtcaa cctgactcga ggcgcgccac ttctaaataa gcgaatttct tatgatttat
      541 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
      601 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
      661 ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag
      721 ggataacagg gtaatataga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca
      781 gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat
      841 gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca
      901 tacattttga tggccgcacg
//
