LOCUS       sequence-62065-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTF270 (pFA6a-PSc-6xGly-3xFlag-KanMX)
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..65
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             90..155
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      184..371
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(239..263)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        446..789
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(857..876)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cttcgtacgc tgcaggtcga cggatccccg ggttaattaa cttggaagta ttgttccaag
       61 gtccaggtgg aggcggtgga ggctctagag actacaagga ccacgacggt gactacaagg
      121 accacgacat tgactacaag gatgacgatg acaagtgact cgaggcgcgc cacttctaaa
      181 taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt
      241 gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac
      301 tctttcctgt aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac
      361 acctctaccg gcagatccgc tagggataac agggtaatat agatctgttt agcttgcctc
      421 gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct
      481 tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc
      541 ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta
      601 cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg
      661 tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt
      721 ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca
      781 taaacaacca tgggtaagga aaagactcac gtttcgaggc cgcgattaaa ttccaacatg
      841 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
      901 atctatcgat tgtatgggaa gcccgatgcg ccaga
//
