LOCUS       sequence-62070-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pZC09 (pFA6a-TEV-6xGly-PrtA-His3MX)
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..58
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             119..292
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             293..466
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     terminator      525..712
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(580..604)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gtacgctgca ggtcgacgga tccccgggtt aattaacgag aatttatact tccaaggttc
       61 tggtggaggc ggtggaggct ctagaaaaac cgcggctctt gcgcaacacg atgaagccgt
      121 ggacaacaaa ttcaacaaag aacaacaaaa cgcgttctat gagatcttac atttacctaa
      181 cttaaacgaa gaacaacgaa acgccttcat ccaaagttta aaagatgacc caagccaaag
      241 cgctaacctt ttagcagaag ctaaaaagct aaatgatgct caggcgccga aagtagacaa
      301 caaattcaac aaagaacaac aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa
      361 cgaagaacaa cgaaacgcct tcatccaaag tttaaaagat gacccaagcc aaagcgctaa
      421 ccttttagca gaagctaaaa agctaaatgg tgctcaggcg ccgaaagtag acgcgaattc
      481 cgcggggaag tcaacctgac tcgaggcgcg ccacttctaa ataagcgaat ttcttatgat
      541 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
      601 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt
      661 tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg
      721 ctagggataa cagggtaata tagatctgtt tagcttgcct cgtccccgcc gggtcacccg
      781 gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg
      841 gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca
      901 tccatacatt ttgatggc
//
