LOCUS       sequence-62268-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a luciferase gene disrupted by an AtCRU3 TALEN Target 494 
            site and a frame-shift mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  TITLE     Plasmids to assay for nuclease cleavage in planta
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGreenII Tfs-494::LUC
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(177..195)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttcatttgg agaggacagc ccaagctcat gtctagacct gcaaggacaa caacaaggcc
       61 aaccatggca aggacgacag ggacaacaag gccaaccatg ggaaggactc gaggaagacg
      121 ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag
      181 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
      241 atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg
      301 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
      361 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
      421 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta
      481 ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc
      541 caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
      601 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag
      661 agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc
      721 tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag
      781 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
      841 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
      901 taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa
      961 g
//
