LOCUS       sequence-62319-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: IV-TCC
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        15..33
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     15..32
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(501..520)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(502..520)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(527..544)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(527..543)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(536..558)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(771..790)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gattacgcca agctatttag gtgacactat agaatactca agctatgcat caagcttggt
       61 accgagctcg gatccactag taacggccgc cagtgtgctg gaattcgccc ttatatagat
      121 gccgtcctag cgcgtctcga ctcaccccag agcaggtcgt ggccattgcc tcgaatggag
      181 ggggcaaaca ggcgttggaa accgtacaac gattgcttcc ggtgctgtgc caagcgcacg
      241 gcctcacccc agagcaggtc gtggcgatcg caagccacga cggaggaaag caagccttgg
      301 aaacagtaca gaggctgttg cctgtgctgt gccaagcgca cggattaacc ccagagcagg
      361 tcgtggcgat cgcaagccac gacggaggaa gacccgcact ggagagagac gtgggcacag
      421 gaaagatact taagggcgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg
      481 catctagagg gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
      541 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      601 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      661 tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      721 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      781 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatc
//
