LOCUS       sequence-62349-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   D'Alessio A, Kluger MS, Li JH, Al-Lamki R, Bradley JR, Pober JS
  TITLE     Targeting of tumor necrosis factor receptor 1 to low density plasma 
            membrane domains in human endothelial cells.
  JOURNAL   J Biol Chem. 2010 Jul 30;285(31):23868-79. doi: 
            10.1074/jbc.M110.122853. Epub 2010 May 28.
  PUBMED    20511226
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.122853"; journalName: "J Biol Chem"; date: 
            "2010-07-30- 30"; volume: "285"; issue: "31"; pages: "23868-79"
COMMENT     Sequence Label: pBMN-neo-Flag-TNFR1 L380A Del229-244
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     303..319
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             475..498
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttatatggg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta
       61 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
      121 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt
      181 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct
      241 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg
      301 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta
      361 gactgccgga tccactagta acggccgcca gtgtgctgga attcgccctt catgagggcc
      421 tggatcttct ttctcctttg cctggccggg agggctctgg cagccccact agccgactac
      481 aaggacgacg atgacaagct agccctggtc cctcacctag gggacaggga gaagagagat
      541 agtgtgtgtc cccaaggaaa atatatccac cctcaaaata attcgatttg ctgtaccaag
      601 tgccacaaag gaacctactt gtacaatgac tgtccaggcc cggggcagga tacggactgc
      661 agggagtgtg agagcggctc cttcaccgct tcagaaaacc acctcagaca ctgcctcagc
      721 tgctccaaat gccgaaagga aatgggtcag gtggagatct cttcttgcac agtggaccgg
      781 gacaccgtgt gtggctgcag gaagaaccag taccggcatt attggagtga aaaccttttc
      841 cagtgcttca attgcagcct ctgcctcaat gggaccgtgc acc
//
