LOCUS       sequence-62377-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty backbone for blue-light-regulated expression of a gene of 
            interest. Expression is induced under blue illumination..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Ohlendorf R, Vidavski RR, Eldar A, Moffat K, Moglich A
  TITLE     From dusk till dawn: one-plasmid systems for light-regulated gene 
            expression.
  JOURNAL   J Mol Biol. 2012 Mar 2;416(4):534-42. doi: 
            10.1016/j.jmb.2012.01.001. Epub 2012 Jan 8.
  PUBMED    22245580
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2012.01.001"; journalName: "J Mol Biol"; date: 
            "2012-03-2- 2"; volume: "416"; issue: "4"; pages: "534-42"
COMMENT     Sequence Label: pDawn
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(66..98)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(102..119)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(129..146)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             165..187
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      complement(263..290)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(306..377)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(425..457)
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
ORIGIN
        1 gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg tcgacggagc tcgaattcgg
       61 atccgaccca tttgctgtcc accagtcatg ctagccatat ggctgccgcg cggcaccagg
      121 ccgctgctgt gatgatgatg atgatggctg ctgcccatgg tatatctcct tcttaaagtt
      181 aaacaaaatt atttctagag caaccattat caccgccaga ggtaaaatag tcaacacgca
      241 cggngttact ctagtatata aacgcagaaa ggcccacccg aaggtgagcc agtgtgactc
      301 tagtagagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga
      361 gcctttcgtt ttatttgatg cctggctcta gtagcgatct acactagcac tatcagcgtt
      421 attaagctac taaagcgtag ttttcgtcgt ttgcagcgcc aaacgtctct tcaggccact
      481 gactagcgat aactttcccc acaacggaac aactctcatt gcatgggatc attgggtact
      541 gtgggtttag tggttgtaaa aacacctgac cgctatccct gatcagtttc ttgaaggtaa
      601 actcatcacc cccaagtctg gctatgcaga aatcacctgg ctcaacagcc tgctcagggt
      661 caacgagaat taacattccg tcaggaaagc tcggcttgga gcctgttggt gcggtcatgg
      721 aattaccttc aacctcaagc cagaatgcag aatcactggc ttttttggtt gtgctta
//
