LOCUS       sequence-62383-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Crook NC, Freeman ES, Alper HS
  TITLE     Re-engineering multicloning sites for function and convenience.
  JOURNAL   Nucleic Acids Res. 2011 Aug;39(14):e92. doi: 10.1093/nar/gkr346. 
            Epub 2011 May 17.
  PUBMED    21586584
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-08"; volume: "39(14):e92. doi"; pages: " 
            10.1093/nar/gkr346. Epub 2011 May 17"
COMMENT     Sequence Label: pGPD2
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        363..626
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     520..543
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(711..729)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 atttcaaaga atacgtaaat aattaatagt agtgattttc ctaactttat ttagtcaaaa
       61 aattagcctt ttaattctgc tgtaacccgt acatgcccaa aatagggggc gggttacaca
      121 gaatatataa catcgtaggt gtctgggtga acagtttatt cctggcatcc actaaatata
      181 atggagcccg ctttttaagc tggcatccag aaaaaaaaag aatcccagca ccaaaatatt
      241 gttttcttca ccaaccatca gttcataggt ccattctctt agcgcaacta cagagaacag
      301 gggcacaaac aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc tgcctggagt
      361 aaatgatgac acaaggcaat tgacccacgc atgtatctat ctcattttct tacaccttct
      421 attaccttct gctctctctg atttggaaaa agctgaaaaa aaaggttgaa accagttccc
      481 tgaaattatt cccctacttg actaataagt atataaagac ggtaggtatt gattgtaatt
      541 ctgtaaatct atttcttaaa cttcttaaat tctactttta tagttagtct tttttttagt
      601 tttaaaacac cagaacttag tttcgacgga taaagcttac tagtatcgat aaagaattct
      661 ctagaggatc cctgcagctc gaggtcgacc ccggggatat cattagttat gtcacgctta
      721 cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag acaacctga
//
