LOCUS       sequence-62489-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Fiordalisi JJ, Johnson RL 2nd, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001;332:3-36. doi: 10.1016/s0076-6879(01)32189-4.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2001"; volume: "332:3-36. doi"; pages: " 
            10.1016/s0076-6879(01)32189-4"
COMMENT     Sequence Label: pCGN K-Ras 12V, 188L
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(54..584,585..587,588..620))
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIL"
     CDS             complement(645..671)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(651..677)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     5'UTR           707..746
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     primer_bind     complement(815..839)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(836..874)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 aagaacaatc aagggtcccc aaactcaccc tgaagttctc aggatccgtc gacttacaaa
       61 attacacact ttgtctttga cttctttttc ttctttttac catctttgct catcttttct
      121 ttatgttttc gaatttctcg aactaatgta tagaaggcat catcaacacc ctgtcttgtc
      181 tttgctgatg tttcaataaa aggaattcca taacttcttg ctaagtcctg agcctgtttt
      241 gtgtctactg ttctggaagg caaatcacat ttatttccta ctaggaccat aggtacatct
      301 tcagagtcct taactctttt aatttgttct ctataatggt gaatatcttc aaatgattta
      361 gtattattta tggcaaatac acaaagaaag ccctccccag tcctcatgta ctggtccctc
      421 attgcactgt actcctcttg acctgctgtg tcgagaatat ccaagagaca ggtttctcca
      481 tcaattacta cttgcttcct gtaggaatcc tctattgttg gatcatattc gtccacaaaa
      541 tgattctgaa ttagctgtat cgtcaaggca ctcttgccta cgccaacagc tccaactacc
      601 acaagtttat attcagtcat ggtggatccg ctagaaggtc ctcccaggct ggcatagtca
      661 ggcacgtcat aaggatagct agaagccata cgcgcttcta caaggcgctg gccgaagagg
      721 tgcgggagtt tcacgccacc aagatccccc ggaggctgga tcggtcccgg tgtcttctat
      781 ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact aaacgagctc
      841 tgcttatata gacctcccac cgtacacgcc taccgcc
//
