LOCUS       sequence-62625-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Nevozhay D, Zal T, Balazsi G
  TITLE     Transferring a synthetic gene circuit from yeast to mammalian cells.
  JOURNAL   Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
  PUBMED    23385595
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-02-5"; volume: "4:1451. doi"; pages: " 
            10.1038/ncomms2471"
COMMENT     Sequence Label: pDN-D2irTNG4kwh
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    215..233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    241..259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(255..458)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(284..304)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        459..838
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aagaaagaac aatcaagggt ccccaaactc accctgaagt tctcaggatc gatccggagc
       61 tttttgcaaa agcctaggcc tccaaaaaag cctcttcact acttctggaa tagctcagag
      121 gccctagagg atccccgggt accaagctta agtttaaacg ctagagtccg gaggctggat
      181 cggtcccggt gtcttctatg gaggtcaaaa cagctctcta tcactgatag ggagatctga
      241 tctctatcac tgatagggat gcttatatag acctcccacc gtacacgcct accgcccatt
      301 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
      361 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
      421 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
      481 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
      541 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
      601 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
      661 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
      721 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
      781 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
      841 cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc
      901 ttc
//
