LOCUS       sequence-62678-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G
  TITLE     Negative autoregulation linearizes the dose-response and suppresses 
            the heterogeneity of gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 
            10.1073/pnas.0809901106. Epub 2009 Mar 11.
  PUBMED    19279212
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0809901106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-31- 31"; volume: "106"; issue: "13"; pages: "5123-8"
COMMENT     Sequence Label: pDN-NG1Tt
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..643
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(93..112)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 aaccccggat cctattaaaa tgtctagatt agataaaagt aaagtgatta acagcgcatt
       61 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
      121 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
      181 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
      241 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
      301 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
      361 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
      421 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
      481 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
      541 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
      601 atgcggatta gaaaaacaac ttaaatgtga aagtgggtct taactcgagt aagcttggta
      661 ccgcggctag ctaagatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta
      721 ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat
      781 ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct
      841 tgagaa
//
