LOCUS       sequence-62688-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Reed B, Blazeck J, Alper H
  TITLE     Evolution of an alkane-inducible biosensor for increased 
            responsiveness to short-chain alkanes.
  JOURNAL   J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 
            10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
  PUBMED    22326628
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2012.01.028"; journalName: "J Biotechnol"; date: 
            "2012-04-15- 15"; volume: "158"; issue: "3"; pages: "75-9"
COMMENT     Sequence Label: pZE-PalkB-GFP
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             461..472
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(511..539)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc
       61 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
      121 caaccttacc agagggcgcc ccagctggca attccgacgt cttacccgga agcccgggtt
      181 ttggaggttt ccaatcgtgc cttggcgcag cgacagccct cggtccccca gatagccatt
      241 gatcaccgcc agcatgcttg cggccagctc gtgtttttcc agcagacgac ggagcaaaaa
      301 ctacccgtag gtgtagttgg cgcaagcgtc cgattagctc aggtttaaga tgtcgagagt
      361 gagagtgggc ggcttaactt tctcagttag gcataaaatt acgtcttaaa tctcgtagcg
      421 actaatttaa taaaaattgg agaacaccaa agaattcatt aaagaggaga aaggtaccgc
      481 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      541 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      601 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      661 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      721 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      781 aaagatgacg ggaactacaa gacacgtgct gaa
//
