LOCUS       sequence-62775-e         504 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, McDonald
            ER 3rd, Li MZ, Hannon GJ, Sorger PK, Kirschner MW, Harper JW, 
            Elledge SJ
  TITLE     Anaphase initiation is regulated by antagonistic ubiquitination and 
            deubiquitination activities.
  JOURNAL   Nature. 2007 Apr 19;446(7138):876-81.
  PUBMED    17443180
REFERENCE   2  (bases 1 to 504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2007-04-19"; volume: "446(7138)"; pages: "876-81"
COMMENT     Sequence Label: pFS331-USP44-catDead-HIS
FEATURES             Location/Qualifiers
     source          1..504
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    125..259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(162..181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     216..235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cctcactaaa gggaacaaaa gctgggtacc gggcccaatg cattggcgcc gcggccgcga
       61 attaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
      121 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
      181 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
      241 atcttatcat gtctggatct acgtaatacg actcactata gttctagagg cgcgcctcag
      301 ctaaggattt cattagacga ggtatcagcg tcttcattgg gatgttggct ccccaacagg
      361 agctctggag gcaaaagttt agaatgtcca ttctcagtaa ctcgttgggt ataaaacaag
      421 atataagctt gagccttgca tacttcgtcc atagtgcaca tgcttagttt ggaatcattg
      481 cagtgtaccc agaaccctcc ttca
//
