LOCUS       sequence-62843-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Blazeck J, Liu L, Redden H, Alper H
  TITLE     Tuning gene expression in Yarrowia lipolytica by a hybrid promoter 
            approach.
  JOURNAL   Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 
            10.1128/AEM.05763-11. Epub 2011 Sep 16.
  PUBMED    21926196
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.05763-11"; journalName: "Appl Environ Microbiol"; date:
            "2011-11"; volume: "77"; issue: "22"; pages: "7905-14"
COMMENT     Sequence Label: pUC-UAS1B2-Leum
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(368..385)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(368..384)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(377..399)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(593..612)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     712..734
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(772..790)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttagtttcg ggttccattg tggatgtgtg tggttgtatg tgtgatgtgg tgtgtggagt
       61 gaaaatctgt ggctggcaaa cgctcttgta tatatacgca cttttgcccg tgatcagtgc
      121 atgcccggat cgaggtgggc gggaacaacg gtctcgataa tgtacgtaca ctacacacaa
      181 agagaagcaa gtgggggcgg gactgcacgg cacttgtgag acacctcagg tcgacccgga
      241 tcgaggtggg cgggaacaac ggtctcgata atgtacgtac actacacaca aagagaagca
      301 agtgggggcg ggactgcacg gcacttgtga gacacctcag ggatccccgg gtaccgagct
      361 cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      421 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      481 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
      541 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
      601 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
      661 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      721 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
      781 tacgcctatt t
//
