LOCUS       sequence-62852-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTF257 (pFA6a-TEV-6xGly-His3MX)
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(85..282)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(576..590)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 cgcataggcc actagtggat ctgatatcat cgatgaattc gagctcgttt aaactggatg
       61 gcggcgttag tatcgaatcg acagcagtat agcgaccagc attcacatac gattgacgca
      121 tgatattact ttctgcgcac ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa
      181 aatataaatc acgctaacat ttgattaaaa tagaacaact acaatataaa aaaactatac
      241 aaatgacaag ttcttgaaaa caagaatctt tttattgtca gtacttcaca tcaaaacacc
      301 tttggttgag ggaacgtcat tggtgccatt gctagaaata gcttctctta tggcaacagc
      361 caaagccttg aacgcactct cacttctgtg gtgatcgttg aaacctctca gacaatcaac
      421 atgcaaagta attctggccg cctccgcgaa actttccaaa aagtgtggaa tcatttcagt
      481 ggataaatca ccaatcatct ctctcttcaa tccaaggtcg attacagcaa atggtctact
      541 agataaatcg actacggcac gtgatagcgc ctcatccaat ggtgcgaacc cagtaccgaa
      601 tctttttaca ccacggacag cacccattgc ttctttgaac gcttgcccta atgcgatacc
      661 gcaatcttcg gtagtatggt gatcgtcaat gtgcaggtca ccaatacatt caacaataag
      721 agaccaacca gagtgttttg ccaacgcatg gatcatatga tccaaaaagc caacacctgt
      781 gtgaatatcg atgacctgtg actgagtagc ttgggaagct acatcgtcat ccttctttgc
//
