LOCUS       sequence-62861-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Hafner M, Max KE, Bandaru P, Morozov P, Gerstberger S, Brown M, 
            Molina H, Tuschl T
  TITLE     Identification of mRNAs bound and regulated by human LIN28 proteins 
            and molecular requirements for RNA recognition.
  JOURNAL   RNA. 2013 May;19(5):613-26. doi: 10.1261/rna.036491.112. Epub 2013 
            Mar 12.
  PUBMED    23481595
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.036491.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "613-26"
COMMENT     Sequence Label: pFRT/FLAG/HA-DEST LIN28B
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..98
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    141..165
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta
       61 agcttggtac catggactac aaggacgacg atgacaagta cccttatgac gtgcccgatt
      121 acgctttgaa ccagatatca acaagtttgt acaaaaaagc aggcttcatg gccgaaggcg
      181 gggctagcaa aggtggtgga gaagagcccg ggaagctgcc ggagccggca gaggaggaat
      241 cccaggtttt gcgcggaact ggccactgta agtggttcaa tgtgcgcatg ggatttggat
      301 tcatctccat gataaaccga gagggaagcc ccttggatat tccagtcgat gtatttgtac
      361 accaaagcaa actattcatg gaaggattta gaagcctaaa agaaggagaa ccagtggaat
      421 tcacatttaa aaaatcttcc aaaggccttg agtcaatacg ggtaacagga cctggtggga
      481 gcccctgttt aggaagtgaa agaagaccca aagggaagac actacagaaa agaaaaccaa
      541 agggagatag atgctacaac tgtggtggcc ttgatcatca tgctaaggaa tgtagtctac
      601 ctcctcagcc aaagaagtgc cattactgtc agagcatcat gcacatggtg gcaaactgcc
      661 cacataaaaa tgttgcacag ccacccgcga gttctcaggg aagacaggaa gcagaatccc
      721 agccatgcac ttcaactctc cctcgagaag tgggaggcgg gcatggctgt acatcaccac
//
