LOCUS       sequence-62879-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy40
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             72..101
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(105..110,111..134,135..170,171..194)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             198..242
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             249..326
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             333..368
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaga gcatcatcat catcaccacc accaccacca ctgaagcgct tggagccacc
      121 cgcagttcga aaaaggtgga ggttctggcg gtggatcggg aggttcagcg tggagccacc
      181 cgcagttcga gaaatagaaa gaaaccgctg ctgctaaatt cgaacgccag cacatggaca
      241 gctagatgaa gcggcggtgg aagaaaaact ttatcgccgt gtccgccgcc aaccggttca
      301 agaagatcag cagctctggc gccctgctgt gagaggacca ggtggacccc cggctgatcg
      361 atggcaaatg aggcagcggc aagatcctgc acaaccagaa cgtgaacagc tggggcccca
      421 tcaccgtgac ccctaccacc gacggcggcg agacaagatt cgacggccag atcatcgtgc
      481 agatggaaaa cgaccccgtg gtggccaagg ccgctgccaa tctggccggc aagcacgccg
      541 agagcagcgt ggtggtgcag ctggacagcg acggcaacta ccgggtggtg tacggcgacc
      601 ccagcaagct ggacggcaag ctgcggtggc agctggtggg acacggcagg gaccacagcg
      661 agacaaacaa cacccggctg agcggctaca gcgccgacga gctggccgtg aagctggcca
      721 agttccagca gagcttcaac caggccgaga acatcaacaa caagcccgac cacatcagca
      781 tcgtgggctg cagcctggtg tccgacgaca agcagaaggg cttcggccac cagttcatca
      841 acgctatgga cgcc
//
