LOCUS       sequence-62882-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy29
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             87..104
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
       61 gcggatctcg gtccgaaacc atgaaacatc accatcacca tcaccccatg aaaatcgaag
      121 aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc gctgaagtcg
      181 gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg gataaactgg
      241 aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc ttctgggcac
      301 acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc ccggacaaag
      361 cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac ggcaagctga
      421 ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat ctgctgccga
      481 acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa gcgaaaggta
      541 agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg attgctgctg
      601 acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg
      661 ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca
      721 tgaatgcaga caccgattac tccatcgca
//
