LOCUS       sequence-62885-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy11
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(247..270)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(280..309)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 gtgcactggc cagggggatc accatccgtc gcccgggcat gtcaataata ccactctgta
       61 catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgaatca
      121 ttaccttctt acgttacaag tataacacaa agtttttatg ttgtcaactg cagtttgggc
      181 tgcaggaatt cgatatcaag cttgtcgacg gagctcgaat tcggatccgg tacctcatta
      241 gtccatgggc ccctggaaca gaacttccag accagaagag tgatggtggt gatggtggtg
      301 atggtggtgt gccatggtca cttggttgtt cacgatcttg tcgccgccag tgtcaacttg
      361 caactgaaac aatatccaac atgaacgtca atttatactg ccctaatggc gaacacgata
      421 acaatatttc ttttattatg ccctctaaaa ccaacgcggt tatcgtttat ttattcaaat
      481 tagatataga acatccgccg acatacaatg ttaatgcaaa aacgcgtttg gtgagcggat
      541 acgaaaacag tcggccgata aacattaatc tgaggtcgat aacaccgtcc ttgaacggaa
      601 cacgagggac gtacgtgatc agctgcattc gcgcgccgcg cctttatcga gatttatttg
      661 catacaacaa gtacactgcg ccgttgggat ttgtggtaac gcgcacacat gcagagctgc
      721 aagtgtggca cattttgtct gtgcgcaaaa cctttgaagc caaaagtacg aggtccgtta
      781 cgggcatgct agcgcacacg gacaatggac ccgacaaatt ctacgccaag gatttaatga
      841 taatgtcggg caacgtatcc gttcatttta tca
//
