LOCUS       sequence-62886-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy27
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             123..131
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(247..270)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(280..300)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     promoter        complement(361..452)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(419..436)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(804..809,810..826,827..832)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta
       61 catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgaatca
      121 ttacctcctt acgttacaag tataacacaa agtttttatg ttgtcaactg cagtttgggc
      181 tgcaggaatt cgatatcaag cttgtcgacg gagctcgaat tcggatccgg tacctcatta
      241 gtccatgggc ccctggaaca gaacttccag accagaagaa tgatgatggt gatggtgatg
      301 tttcatggtt tcggaccgag atccgcgccc gatggtggga cggtatgaat aatccggaat
      361 atttataggt ttttttatta caaaactgtt acgaaaacag taaaatactt atttatttgc
      421 gagatggtta tcattttaat tatctccatg atctattaat attccggagt atacgtagcc
      481 aaccactaga actatagcta gagtcctggg cgaacaaacg atgctcgcct tccagaaaac
      541 cgaggatgcg aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag
      601 gtcttccgat ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca
      661 gcaaggccgc caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc
      721 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga
      781 aacggatgaa ggcacgaacc cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca
      841 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
      901 gcgca
//
