LOCUS       sequence-63127-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Westermann B, Neupert W
  TITLE     Mitochondria-targeted green fluorescent proteins: convenient tools 
            for the study of organelle biogenesis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2000 Nov;16(15):1421-7.
  PUBMED    11054823
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2000-11"; volume: "16(15)"; pages: "1421-7"
COMMENT     Sequence Label: pYX142-mtGFP
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             235..948
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(265..293)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     888..908
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aagcttatgg cctccactcg tgtcctcgcc tctcgcctgg cctcccagat ggctgcttcc
       61 gccaaggttg cccgccctgc tgtccgcgtt gctcaggtca gcaagcgcac catccagact
      121 ggctcccccc tccagaccct caagcgcacc cagatgacct ccatcgtcaa cgccaccacc
      181 cgccaggctt tccagaagcg cgcctactct tccggatccc cgggtaccag atctatgagt
      241 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      301 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      361 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
      421 actttcactt atggtgttca atgcttttca agatacccag atcatatgaa acagcatgac
      481 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
      541 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      601 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
      661 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
      721 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
      781 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
      841 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
      901 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata agaattc
//
