LOCUS       sequence-63276-e        5484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Crefcoeur RP, Yin R, Ulm R, Halazonetis TD
  TITLE     Ultraviolet-B-mediated induction of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800.
  PUBMED    23653191
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1779. doi"; pages: " 
            10.1038/ncomms2800"
COMMENT     Sequence Label: pCMV-AD-uvr8at
FEATURES             Location/Qualifiers
     source          1..5484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             667..687
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             715..1257
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     primer_bind     complement(2661..2680)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2662..2680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2954..3075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2991..3010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3045..3064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3082..3537)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3219..3240)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3431..3450
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3564..3668
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(join(3758..4549,4550..4618))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4381..4400
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4788..5376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5277..5296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 atgggcccta aaaagaagcg taaagtcgcc atcgatcagc tcaccatggt gtttccttct
      721 gggcagatct caaaccaggc cctggcctta gcaccgtcct ctgccccagt ccttgcccag
      781 accatggtcc cttcctcagc catggtacct ctggctcagc ccccagctcc tgccccagtt
      841 ctaaccccgg gtcctcccca gtccctgtct gcacctgttc caaagagcac ccaggctggg
      901 gaaggcacgc tgtcggaagc cctgctgcac ctgcagtttg atgctgatga agacttgggg
      961 gccttgcttg gcaacagcac agacccagga gtgttcacag acctggcatc tgtggacaac
     1021 tcagagtttc agcagctcct gaaccagggt gtgtccatgt ctcactccac agctgagccc
     1081 atgctgatgg agtaccctga agctataact cgcctggtga cagggtccca gaggccccct
     1141 gacccagctc ccacacccct ggggacctcg gggcttccca atggtctctc cggagatgaa
     1201 gacttctcct ccattgcgga catggacttc tctgctctgc tgagtcagat cagctccagc
     1261 ggccaaggat ccgcggagga tatggctgcc gacgaagtta cggctcctcc tcgtaaggtt
     1321 cttatcatct ccgctggtgc tagccactcc gtcgctcttc tctctggtga cattgtttgt
     1381 tcttggggtc gaggagagga tggacagtta ggtcatggcg atgcagagga tcgaccttct
     1441 ccgactcagc ttagcgcttt agatggccac caaattgttt ccgttacctg tggtgctgat
     1501 cacactgttg cttattcaca atcaggcatg gaagtctaca gttggggatg gggtgatttt
     1561 gggagattag gccatggtaa ctcaagcgac ttgtttactc cgctaccaat caaagcattg
     1621 cacggtattc ggatcaagca gattgcttgt ggggatagtc attgtttggc tgtcactatg
     1681 gaaggagagg tccagagttg gggccgcaac cagaatggtc aacttggtct gggggacacc
     1741 gaagattctc tagtgcctca gaagattcaa gcctttgagg gaataccaat caaaatggtt
     1801 gctgctggtg cagaacacac tgctgcagtt acagaagatg gtgacctcta tggatggggc
     1861 tggggaagat acggaaattt gggattaggt gaccggactg accgcttagt tcctgaaaga
     1921 gttacctcta ctggtggtga gaaaatgtca atggttgctt gtggatggcg gcacacaata
     1981 tcagtttcct actctggagc attgtatact tatggatgga gcaaatatgg acagctagga
     2041 catggagact tggaggatca ccttattcct cacaaactgg aagcactgag caacagtttt
     2101 atctcccaga tttcgggagg ttggagacat acaatggcat tgacttcaga tggaaaacta
     2161 tatggatggg gttggaataa gtttggacaa gtaggagtcg gcaataattt agatcagtgt
     2221 tctcctgtgc aagtgcgatt tcccgatgat cagaaagtag ttcaagtctc atgtggatgg
     2281 agacatacct tggctgtcac tgaaagaaat aacgtgtttg cttggggtag aggtacaaat
     2341 ggacagctcg gcattggaga gtcggttgac aggaactttc ccaagattat agaggcactg
     2401 agcgtcgatg gagcaagtgg acaacatata gaatcttcta atatcgatcc atcttcaggg
     2461 aaaagctggg tgtcgcctgc agagagatat gcagttgttc ctgatgaaac gggcctaacg
     2521 gatggttcaa gcaaaggtaa tggaggtgat atcagtgttc cacaaactga tgtcaagcgt
     2581 gtacgaattt aagcggccgc gtcgacctct agactgcagc tcgagggggg gcccggtacc
     2641 aggtaagtgt acccaattcg ccctatagtg agtcgtatta caattcactc gatcgccctt
     2701 cccaacagtt gcgcagcctg aatggcgaat ggagatccaa tttttaagtg tataatgtgt
     2761 taaactactg attctaattg tttgtgtatt ttagattcac agtcccaagg ctcatttcag
     2821 gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat ttgtagaggt
     2881 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     2941 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3001 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3061 catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     3121 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     3181 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     3241 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     3301 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     3361 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     3421 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     3481 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     3541 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3601 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3661 ggaagaatcc tgaggccggg ccataacttc gtataatgta tcgtatacga agttatccat
     3721 gggccccccc tcgacatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3781 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3841 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3901 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3961 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4021 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4081 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4141 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4201 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4261 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4321 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4381 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4441 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4501 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4561 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4621 tcttcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4681 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4741 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4801 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4861 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4921 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4981 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5041 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5101 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5161 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5221 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5281 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5341 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5401 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5461 tctgtggata accgtattac cgcc
//
