LOCUS       sequence-63280-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Voytas Golden Gate TALEN assembly 
            incorporating a CAG promoter for mammalian expression and a T7 
            promoter for synthesis of TALEN mRNA. Uses homodimeric FokI 
            domains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Hermann M, Cermak T, Voytas DF, Pelczar P
  TITLE     Mouse genome engineering using designer nucleases.
  JOURNAL   J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930.
  PUBMED    24747757
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-04-2"; volume: "(86). doi"; pages: " 
            10.3791/50930"
COMMENT     Sequence Label: pCAG-T7-TALEN(Sangamo)-Destination
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(17..35)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(45..62)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(45..61)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(54..76)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(637..657)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(677..695)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
       61 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      121 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      181 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cccgtctcca ggggggcacc
      241 cgtcagtgca ttgcgcgatg catgcactgc ctccattgcg gtcacgccgc cacgttttgc
      301 aatcttcaca agttggcctg tgtccaactg taacggcgga cctctcaact cccccgcatc
      361 cgtgagcaag gcctccaggg cgcgtgcgcc ggaccactgt ttgccgacgc caacgatgtc
      421 ttcgtgtgtc gcctctggca acgccgtgat tatgtgctga tacgtgacag cgacggtccc
      481 taacgctgcc gggtgttggc tgagcgcaac gatgtgcgcg tgtgtaaacc catggcccac
      541 cagtgcctcg tggtgctgcg ccactgtcga acgcaccttc ggtttgatct tctcttgctg
      601 ctgctgactg tagccgagcg tgcgtagatc cacctgcacc ttcctctttt tcttaggaga
      661 tctcatggtg gcggccccta tagtgagtcg tattattaat taaggatctg taggaaaaag
      721 aagaaggcat gaacatggtt agcagaggct ctagagccgc cggtcacacg ccagaa
//
