LOCUS       sequence-63314-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-RK-GFP-mIRBP156-CHER-WPRE
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    10..62
                     /label=MCS
                     /note="multiple cloning site"
     CDS             374..388
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     regulatory      395..404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(546..564)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 taagatatcc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
       61 ccctacacag acatggctca gcaagatgag atgcaatgtt ccttccgggt caaaccaccc
      121 tggcctcttc ttcagagtcc agctcatgtg cttgaattag acaggattaa aggcttagca
      181 gagctggaag cctcacatct aactcccaca ttgagcccca gaccttctgt ctgcctgcta
      241 agaagggcaa gggagagagg cagctgcatg accacagcct tgtacacacg gagctcttat
      301 ccaggagcca aggctctggg atccaccggt cgccgctagc gttcgacccg ggatcgatag
      361 tttaaacctg tacgacgatg acgataagga tcccgccacc atggtgagca agggcgagga
      421 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
      481 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      541 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
      601 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
      661 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      721 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
      781 gaagctgcgc g
//
