LOCUS       sequence-63386-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  TITLE     Trono Lab Misc Plasmids
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRRLSIN.cPPT.PGK-GFP.WPRE
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(51..561)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(114..132)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    607..724
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtggatcccc ctggggagag
       61 aggtcggtga ttcggtcaac gagggagccg actgccgacg tgcgctccgg aggcttgcag
      121 aatgcggaac accgcgcggg caggaacagg gcccacacta ccgccccaca ccccgcctcc
      181 cgcaccgccc cttcccggcc gctgctctcg gcgcgccctg ctgagcagcc gctattggcc
      241 acagcccatc gcggtcggcg cgctgccatt gctccctggc gctgtccgtc tgcgagggta
      301 ctagtgagac gtgcggcttc cgtttgtcac gtccggcacg ccgcgaaccg caaggaacct
      361 tcccgactta ggggcggagc aggaagcgtc gccggggggc ccacaagggt agcggcgaag
      421 atccgggtga cgctgcgaac ggacgtgaag aatgtgcgag acccagggtc ggcgccgctg
      481 cgtttcccgg aaccacgccc agagcagccg cgtccctgcg caaacccagg gctgccttgg
      541 aaaaggcgca accccaaccc cgtggaattc gatatcaagc ttctcgaggc tagtctcgtg
      601 atcgataaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
      661 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
      721 aaaagttaac cgataccgtc gagatccgtt cactaatcga atggatctgt ctctgtctct
      781 ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg
      841 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
      901 aaaaactatt cttaaaccta ccaagcctcc tactatcatt
//
