LOCUS       sequence-63387-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     Trono Lab Misc Plasmids
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRRLSIN.cPPT.PGK-GFP.WPRE
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    44..632
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(97..117)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(515..526)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtaaag cggccgcgtc gacaatcaac ctctggatta
       61 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
      121 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
      181 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
      241 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
      301 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
      361 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
      421 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
      481 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
      541 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
      601 gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga
      661 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
      721 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
      781 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      841 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      901 ggtaacta
//
