LOCUS       sequence-63435-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of DsRed in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ
  TITLE     Global protein stability profiling in mammalian cells.
  JOURNAL   Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489.
  PUBMED    18988847
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2008-11-7- 7"; volume: "322"; issue: 
            "5903"; pages: "918-23"
COMMENT     Sequence Label: MSCV-CMV-DsRed-IRES-d1EGFP
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..31
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      185..194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(321..339)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(371..391)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     589..608
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     783..806
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gcctgttttg
       61 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
      121 cggacctgca ggaattgatc cgcggccgca ccggctagcg ctaccggact cagatccacc
      181 ggtcgccacc atggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg
      241 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      301 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
      361 ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc
      421 cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      481 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggctc
      541 cttcatctac aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtaatgca
      601 gaagaagact atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
      661 gaagggcgag atccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
      721 caagtccatc tacatggcca agaagcccgt gcagctgccc ggctactact acgtggactc
      781 caagctggac atcacctccc acaacgagga ctacaccatc gtgga
//
