LOCUS       sequence-63530-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Bernardos RL, Barthel LK, Meyers JR, Raymond PA
  TITLE     Late-stage neuronal progenitors in the retina are radial Muller glia
            that function as retinal stem cells.
  JOURNAL   J Neurosci. 2007 Jun 27;27(26):7028-40.
  PUBMED    17596452
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2007-06-27"; volume: "27(26)"; pages: "7028-40"
COMMENT     Sequence Label: gfap:GFP-NUC
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..56
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             60..80
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             84..104
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    247..368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(284..303)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     338..357
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(375..830)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(512..533)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     724..743
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agctgtacaa gtccggactc agatcccgag ctgatccaaa aaagaagaga aaggtagatc
       61 caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtaggatcc accggatcta
      121 gataagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
      181 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      241 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      301 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
      361 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
      421 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
      481 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
      541 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
      601 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
      661 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
      721 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
      781 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
      841 ttttcgggga a
//
