LOCUS       sequence-63543-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy48
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(703..738)
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             complement(745..822)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             complement(829..873)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgctcattcc aggccctggg cgtcccagct cagggacacc ttgttgtttt cggccttctg
      121 cacccagtcg ccattggcgt ccttggtgtg ctttctgccg gcctcgtcca cggccagttc
      181 gctagaccgc acggacacgt ccacccgcag gccgttggcg tccatagcgt tgatgaactg
      241 gtggccgaag cccttctgct tgtcgtcgga caccaggctg cagcccacga tgctgatgtg
      301 gtcgggcttg ttgttgatgt tctcggcctg gttgaagctc tgctggaact tggccagctt
      361 cacggccagc tcgtcggcgc tgtagccgct cagccgggtg ttgtttgtct cgctgtggtc
      421 cctgccgtgt cccaccagct gccaccgcag cttgccgtcc agcttgctgg ggtcgccgta
      481 caccacccgg tagttgccgt cgctgtccag ctgcaccacc acgctgctct cggcgtgctt
      541 gccggccaga ttggcagcgg ccttggccac cacggggtcg ttttccatct gcacgatgat
      601 ctggccgtcg aatcttgtct cgccgccgtc ggtggtaggg gtcacggtga tggggcccca
      661 gctgttcacg ttctggttgt gcaggatctt gccgctgcct catttgccat cgatcagccg
      721 ggggtccacc tggtcctctc acagcagggc gccagagctg ctgatcttct tgaaccggtt
      781 ggcggcggac acggcgataa agtttttctt ccaccgccgc ttcatctagc tgtccatgtg
      841 ctggcgttcg aatttagcag cagcggtttc ttt
//
